Thanks for stopping by.I am trying to process 50 images from.nd2 to.TIF, but the exported images are not what I expect and I'm not sure what is wrong. My.nd2's have two channels and I would like the final.TIF to be an image of both channels. However, the.TIF output of my code is an image of just one channel. SetBatchMode(true); //Batch mode processing setting in ImageJfor (i=0; i.
ImageJ is an image processing and analysis application written in Java, widely used in the life sciences fields. ImageJ Tutorial is a beginners guide to using ImageJ. Loci Tools is a ImageJ plugin for opening Nikon, Olympus, Zeiss and Leica Confocal files as well as other fomarts in ImageJ. To install, place the.jar file into the plugins.
Hello,I am trying to open images in image J that were saved as ND2 files taken on a Nikon Eclipse Ti. The files are opening up in image j as stacks of images on top of each other. I was wondering if there was a way to either convert all of the ND2 files to.tif without going back to the scope and re-saving as different files, or if there was a way to fix the files in image j so they are back to one channel with RGB. Just as a note, these images were not taken as stacks. They were taken as 2D images.Thank you,Leonie. Hi,Do you mean the images open as z stacks rather than composite (multichannel)?If you want to preserve the channels as separate (essential for quantitation), you can do Image Hyperstacks Re-order Hyperstack and choose Channels Slices and Slices Channels as below. Then your “z stack” will become a multi-channel image.You can fix the colors using Image Color Channels Tool.I would not use the “RGB Color” conversion if I was going to analyze the intensity or particles later.Hope this helps.Theresa.